T62D mutation confers DA leak. Representative amperometric current obtained
from hDAT (A), T62A-hDAT (B), and T62D-hDAT (C). Cells were preloaded by
incubating them in a bath solution containing 1 μM DA. Cells were then
washed, and DA leak was estimated by measuring the decrease in amperometric
current upon cocaine application. D, DA leak from hDAT (n = 8),
T62A-hDAT (n = 7), and T62D-hDAT (n = 12) cells reported as
mean ± S.E.M. The dotted horizontal lines represent the amperometric
currents recorded when all dopamine transporters are blocked by cocaine.
*, one-way ANOVA, p < 0.001; in Bonferroni post tests,
p < 0.01 for T62D-hDAT versus T62A-hDAT and hDAT.