Fig. 3.

Western immunoblot analysis of subcellular localization of CYP1A1 proteins. MT and ER (i.e., microsomes) fractions (A) and cytosolic fractions (B) were isolated and resolved by SDS-PAGE or mixed-alcohol-detergent in PAGE. Designation of the mouse lines, with or without TCDD or BNF treatment, noted at the top, is the same as that in Fig. 2. Across each row, CYP1A1, POR (ER marker), PHB (MT marker), and GCLM (cytosolic marker) proteins were detected using antibodies. Protein loadings (micrograms per lane) for the CYP1A1 immunoblots are noted in parentheses; 20 μg of protein from subcellular fractions was loaded for the detection of the ER and MT markers; 30 μg of protein was loaded for detection of the cytosolic marker. K, kidney; Liv, liver; S.I., small intestine. Lg, lung.