TABLE 1.
Effect of oral BaP on body weight, organ weight, liver enzymes, hematocrit, hemoglobin, and peripheral blood cell types
Mice (n = 4 per group) were fed corn oil-soaked food or BaP-soaked food (125 mg/kg/day) for 18 days. Values are expressed as means ± S.E.M.
|
Cyp1a1
|
Corn Oil Only
|
BaP
|
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|---|---|---|---|---|---|---|---|---|
| WT | mc | mtp | KO | WT | mc | mtp | KO | |
| Body weight (g, gained or lost) | +0.8 ± 0.5 | +0.6 ± 0.3 | +1.6 ± 0.5 | +1.0 ± 0.4 | +0.7 ± 0.2 | +1.1 ± 0.3 | −1.3 ± 0.3* | −2.7 ± 0.5* |
| Liver weight (mg/g body weight) | 47 ± 4.1 | 46 ± 2.0 | 47 ± 0.9 | 47 ± 2.2 | 41 ± 1.1 | 42 ± 1.3 | 53 ± 2.2* | 55 ± 2.3* |
| Spleen weight (mg/g body weight) | 3.8 ± 0.2 | 3.9 ± 0.1 | 3.6 ± 0.1 | 3.5 ± 0.1 | 3.4 ± 0.1 | 3.1 ± 0.2 | 2.0 ± 0.1* | 1.2 ± 0.1*† |
| Thymus weight (mg/g body weight) | 1.6 ± 0.3 | 1.6 ± 0.1 | 1.6 ± 0.4 | 2.3 ± 0.4 | 1.5 ± 0.1 | 1.5 ± 0.2 | 0.5 ± 0.04* | 0.2 ± 0.04*† |
| ALT (IU/l) | 33 ± 6.9 | 26 ± 6.1 | 18 ± 3.2 | 21 ± 2.1 | 21 ± 4.2 | 41 ± 3.1 | 48 ± 5.1* | 53 ± 3.7* |
| AST (IU/l) | 105 ± 18 | 115 ± 16 | 105 ± 16 | 81 ± 6.8 | 76 ± 1.9 | 84 ± 16 | 98 ± 1.5* | 93 ± 1.8* |
| Hematocrit (%) | 52 ± 1.0 | 52 ± 0.5 | 53 ± 1.1 | 52 ± 0.5 | 53 ± 2.8 | 52 ± 1.1 | 45 ± 1.2* | 38 ± 1.0*† |
| Total hemoglobin (mM) | 6.9 ± 0.6 | 7.3 ± 0.3 | 7.3 ± 0.4 | 6.8 ± 0.3 | 6.9 ± 0.2 | 7.2 ± 0.6 | 6.2 ± 0.3* | 6.1 ± 0.1* |
| Methemoglobin (%) | 2.2 ± 0.9 | 1.0 ± 0.2 | 1.2 ± 0.5 | 1.4 ± 0.4 | 1.3 ± 0.2 | 0.9 ± 0.2 | 2.4 ± 0.2* | 4.9 ± 0.5* |
| Peripheral blood | ||||||||
| Neutrophils (%) | 24 ± 4.7 | 24 ± 1.8 | 21 ± 4.0 | 22 ± 4.1 | 20 ± 4.2 | 19 ± 3.2 | 43 ± 4.2* | 42 ± 3.3* |
| Lymphocytes (%) | 78 ± 5.3 | 73 ± 5.4 | 77 ± 4.6 | 78 ± 4.4 | 76 ± 4.4 | 82 ± 4.0 | 52 ± 5.7* | 53 ± 2.1* |
*
P <0.05, compared with BaP-treated WT 1A1 wild-type mice.
†
P <0.05, compared with BaP-treated mtp 1A1 mice.