Fig. 3.

Quantification of FAK activation loop Tyr⁵⁷⁶ and Tyr⁵⁷⁷ in MEFs expressing oncogenic Y⁵²⁹FSrc. Shown are amounts of unmodified Tyr⁵⁷⁶/Tyr⁵⁷⁷ (□), mono-pTyr⁵⁷⁶ (), and bis-phosphorylated pTyr⁵⁷⁶/pTyr⁵⁷⁷ () activation loop peptides, and the sum amount of activation loop peptides (▪) from a FAK trypsin digest after immunoprecipitation from confluent monolayers of MEFs expressing oncogenic Y⁵²⁹FSrc. The mounts of each peptide are shown after correction for FAK immunoprecipitation recoveries and normalization to the amount total FAK. Data points represent the means of three separate experiments, where error bars are ± S.E.M.