Fig. 3.

DMI reduces KCl-stimulated CREB phosphorylation in primary cultures of NAc and striatal tissue. The ratio of P-CREB/β-actin was determined for each sample and normalized to the control group ratio to yield a -fold induction. Representative Western blots are shown to the right of each panel. A, a 3-h treatment with KCl (40 mM) significantly increased P-CREB; a 1-h pretreatment with DMI dose dependently decreased KCl-stimulated P-CREB. B, likewise, a 24-h pretreatment with DMI decreased KCl-stimulated P-CREB. ^*, P < 0.05; ^**, P < 0.01 compared with vehicle (Veh). #, P < 0.05; ##, P < 0.01 compared with KCl alone. Fisher's protected t tests, n = 3 to 4 experiments per dose of DMI with treatments given in triplicate.