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. 2009 Jan 13;75(4):733–739. doi: 10.1124/mol.108.053819

TABLE 1.

Fluorescent and bioluminescent protein fragments used in PCA assays

Protein Split Position Ex/Em References
nm
BiFC GFP 157-158 485/500 Ghosh et al., 2000
YFP, Citrine, Venus 154-155; 172-173 515/528 Hu et al., 2002; Shyu et al., 2006
CFP, Cerulean 154-155; 172-173 452/478 Hu et al., 2002; Shyu et al., 2006
Venus/Cerulean 154-155; 172-173 504/513 Hu and Kerppola, 2003; Shyu et al., 2006
mRFP1-Q66T 154-155; 168-169 549/570 Jach et al., 2006
mCherry 159-160 587/610 Fan et al., 2008
BiLC RLuc 110-111 Ch/485 Stefan et al., 2007
GLuc 93-94 Ch/480 Remy and Michnick, 2006

Ex/Em, excitation/emission wavelengths; Ch, coelenterazine h