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. 2009 Jan 14;75(4):744–750. doi: 10.1124/mol.108.053462

Fig. 4.

Fig. 4.

Morphine suppresses reporter activity through MOR1 3′-UTR. A, pSVUTR reporter assay. Luciferase coding region (blank box); MOR1 poly (A) signal (gray box); MOR1 3′-UTR (broken line). N2A-MOR cells were treated with morphine (10-8 to 10-5 M) 3 h before transfected with 500 ng of pSVUTR plasmid; 2 ng of pCMV-Rluc was cotransfected for normalization. The morphine concentration is plotted against the normalized luciferase activity of reporter constructs (firefly luciferase/R. reniformis luciferase) relative to control. The graph shows a single experiment performed in duplicate. The experiment was repeated three times with similar results. Student's t test was performed by comparing each sample to the control. n = 3; *, p < 0.05. B, pSVPA reporter assay. Legends are the same as in A, except for 200 ng of pSVPA used in the transfection. The experiment was repeated three times with similar results. Student's t test was performed by comparing each sample to the control; n = 3.