Fig. 2.
Effect of substitution of Phe436 and Ile443/Leu444 with different hydrophobic residues on α2B-AR transport to the cell surface. A, specific [3H]RX821002 binding on intact HEK293T cells transfected with α2B-AR and its mutants. HEK293T cells cultured on six-well plates were transfected with GFP-conjugated α2B-AR or individual mutants. The cell-surface expression of the receptors was measured by intact cell ligand binding as described under Materials and Methods. The data are presented as the percentage of specific binding from cells transfected with α2B-AR and are presented as the mean ± S.E. of three separate experiments. *, p < 0.05 versus cells transfected with α2B-AR. B, subcellular localization of α2B-AR and its mutants revealed by detecting GFP fluorescence.