Fig. 5.

Induction of anandamide-metabolizing cytochrome P450 enzymes in activated microglial cells. Mouse microglial BV-2 cells were stimulated for 24 h with the treatments indicated. Whole-cell lysates (60 μg protein/lane) from the BV-2 cells were separated on a 4 to 20% SDS-polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes. The membranes were probed with antibodies against either P450 4F (A), P450 3A (B), or β-actin (A and B) followed by horseradish peroxidase-conjugated secondary antibodies. The signals were detected using the enhanced chemiluminescence system, and band densities were quantitated as described under Materials and Methods. Shown are data obtained from individual experiments with a single replicate per treatment and are representative of at least four independently performed experiments.