Suppression of production of SLAP mRNA by inhibitors of signaling pathways. RBL-2H3 cells were exposed to the indicated concentrations of inhibitors for 20 min before addition of 50 ng/ml Ag. SLAP mRNA was determined 2 h after addition of Ag by real time PCR. Data (mean ± SEM) are from one experiment that is representative of two separate experiments. Abbreviations: Bay, Bay 11–7082; PD, PD-98059; SB, SB 202190; SP, SP 600125; LY, LY 294002; Csp.A, cyclosporine; Ro-31, Ro-31 8220.