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. 2009 Jun 2;3(6):e449. doi: 10.1371/journal.pntd.0000449

Figure 2. Recombinant SmCL3 expressed in Pichia pastoris.

Figure 2

(A) SmCL3 after purification by nickel-affinity chromatography was resolved by SDS-PAGE analysis (4–20%) and stained by SimplyBlue SafeStain. Lanes A and B represent recombinant enzyme before and after treatment with Endo-H glycosidase, respectively.