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. 2009 Jun 2;3(6):e449. doi: 10.1371/journal.pntd.0000449

Figure 9. Detection of SmCL3 by Western blot in soluble S. mansoni extracts using mouse polyclonal IgG antibodies.

Figure 9

Recombinant enzyme or soluble S. mansoni protein extracts (each 20 µg) were resolved by SDS-PAGE (15%) and electroblotted onto PVDF membrane. IgG purified antibodies reacted with (1) deglycosylated recombinant protein, (2) glycosylated protein, soluble extracts of (3) adults, (4) eggs and (7) 1 day old in vitro transformed schistosomula. Extracts of (5) miracidia and (6) cercariae did not react.