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Porcine liver esterase-mediated hydrolysis of Q2. The stability of Q2 (70 μM) in the presence of porcine liver esterase (10 units/ml) at 37°C was monitored by reversed-phase HPLC using a Vydac C18 column with an eluent consisting of solvent A (H2O/0.1% TFA) and solvent B (CH3CN/0.1% TFA) with a 30-min gradient consisting of 5 to 50% A, a flow rate of 1 ml/min, and monitoring at 280 nm. Percentage of Q2 remaining was calculated based on the starting amount of Q2.
