Table 1.

Bypass efficiency of 8-oxoG by pols δ and η

	Yeasta						Humanb,c	Mouseb
	δexo+		δexo−		η		η	η
RPA/RFC/PCNA	−	+	−	+	−	+	−	−
Bypass (%)d
3′-CCGATT	8.9	25	4.8	24	47	54	24	17
3′-CCGoATT	1.3	4.1	1.5	5.9	62	69	36	31
Relative bypass (%)	15	16	31	25	130	130	150	180
Switch	No	No	No	No	Yes	Yes	No	No
Bypass (%)
3′-TTGGG	71	75			34	23
3′-TTGoGG	18	23			86	57
Relative bypass (%)	25	31			250	250
Switch	No	No			Yes	Yes

^aEfficiency measurements made using 70-mer template with streptavidin blocked ends (see ‘Materials and Methods’ section).

^bEfficiency measurements made using 45-mer template (see ‘Materials and Methods’ section).

^cBypass efficiency measured in three other template sequence contexts was 3′ … CG_oATC … = 220%; 3′- … CAG_oTC … = 180%; 3′- … CAG_oTT … = 180%.

^dExperiments depicted in Figures 1 and 2 were quantified and the bypass amount on each template calculated as previously described (39,41). Relative bypass is the amount of 8-oxoG bypass compared to undamaged DNA. Switch is defined as a change from lower to higher termination probability when comparing values at the 8-oxoG and next undamaged base.