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. 2009 Mar 12;37(9):2830–2840. doi: 10.1093/nar/gkp103

Table 2.

Dark blue plaque frequency for G and 8-oxoG bypass reactions

−RPA/RFC/PCNA
 +RPA/RFC/PCNA
G 8-oxoG G 8-oxoG
Yeast
    δexo+
        Totala 54 728 2441 29 786 446
        Dark blue 26 705 14 132
        MF (%) 0.05 29 0.05 30
    δexo−
        Total 24 049 866 12 710 3528
        Dark blue 37 210 17 800
        MF (%) 0.15 24 0.13 23
    η
        Total 6869b 5506 5342 7590
        Dark blue 121b 165 92 242
        MF (%) 1.8b 3.0 1.7 3.2
    Human ηc
        Total 4083 3702
        Dark blue 134 1081
        MF (%) 3.3 29
    Mouse η
        Total 4345 3420
        Dark blue 166 1031
        MF (%) 3.8 30

aValues given are the numbers of plaques of each type counted and the resulting mutation frequencies for G/8-oxoG bypass reactions. Data shown is from experiments using a 70-mer template with streptavidin-blocked ends (see ‘Materials and Methods’ section).

bThis data has been previously published (50).

cAdditional experiments using a 45-mer template (see ‘Materials and Methods’ section) gave dark blue plaque frequencies of 32% (3′-CCGoATC), 29% (3′-CCAGoTC) and 32% (3′-CCGoAT T).