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CD of 32R (2 μM) in 50 mM Tris pH 7.4, 100 mM KCl in the presence of increasing amounts of Up1 (r = 0, 1, 4, 6) (r = [protein]/[DNA]) (a); hnRNPA1 r = 0, 1, 4, 6, (b); trypsinogen inhibitor (TI) (r = 0, 1, 4, 6) (c). The CD of Up1 at three concentrations is reported (2, 4 and 8 μM) (d). Spectra have been recorded at room temperature with a path length cuvette of 0.5 cm. Ordinate reports ellipticity values in mdeg.
