Figure 6.

Co-occupancy test of the gene loci identified through AEBP2 and SUZ12 ChIPs. (A) Co-occupancy test of the genes identified from AEBP2 and SUZ12-ChIP sequencing. The left panel indicates the genes derived from each round of ChIP sequencing. Grm8, Abcc3 and Phkb were derived from the AEBP2-ChIP sequencing, while Pax1, Acvrinp1 and A20Rik were from the SUZ12-ChIP sequencing. The three loci (Grm8, Abcc3 and Phkb) were first tested through individual ChIP assays using the AEBP2 antibody, and later using another antibody (SUZ12) for the co-occupancy test. This was also repeated for the SUZ12 set (Middle). A subset of the known Polycomb target loci (Barx1 and Zic1) were also included for the co-occupancy test (bottom). (B) Co-occupancy of AEBP2 and SUZ12 on the HoxA9 locus. The co-occupancy of AEBP2 and SUZ12 was only detected at the 3.75-kb upstream region of the transcription start site of HoxA9. We also performed another independent ChIP using the YY1 antibody to test if YY1 is also involved in the targeting of the PRC2 to these loci.