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. 2009 Mar 20;37(9):3007–3020. doi: 10.1093/nar/gkp179

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Dazap2 interacts with all members of the Tcf/Lef family. (A) The region of TCF-4 required for interaction with Dazap2 as identified by GST pull-down assays (aa 214–228) is partly conserved in other Tcf/Lef family members. The corresponding sequences of the human proteins were aligned using the ClustalV program. Fully conserved amino-acid residues are boxed whilst amino-acid residues with similar biochemical properties are underlined. GenBank accession numbers: TCF-4, NP_110383; TCF-3, NP_112573; LEF-1, NP_057353; TCF-1, NP_003193. (B) Co-immunoprecipitation of Dazap2-FLAG and MYC-tagged TCF/LEF transcription factors in human HEK 293 cells transfected with various Tcf expression constructs as indicated. Co-immunoprecipitation of TCF/LEF proteins using an anti-FLAG antibody is also shown. In the gel denoted ‘Input’, 10% of total lysates was loaded. The asterisks indicate the heavy chains of immunoglobulins used in the experiments.