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. 2009 Mar 18;37(9):2962–2973. doi: 10.1093/nar/gkp180

Figure 6.

Figure 6.

HSF1 is involved in p53-mediated apoptosis (A) SAOS2-tetWTp53 cells seeded into 48-well plates were transfected with indicated siRNAs. Twenty-four hours later cells were split into 96-well plates then treated with 0.1 µg/ml doxycycline (doxy) for 48 h before proliferation was measured using WST-1 assay. (B) Upper panel: Saos2-p53 cells in 12-well plates were treated for 36 h with 0.1 µg/ml doxy (right) or left untreated (left) before analysis of active caspase-3 by flow cytometry. Events in upper region represent active caspase-3 staining. Lower panel: SAOS2-tetWTp53 cells were transfected with the indicated siRNAs. After 48 h cells were treated for a further 36 h with 0.1 µg/ml doxy or left untreated then analyzed as before. Percentage of active Caspase-3 positive cells is shown from one of three experiments (lower panel). Error bars represent standard deviation. (C) Wild-type or HSF−/– MEFs seeded in 6-well plates were treated with 50 µM etoposide or 50 µM actinomycin D for 48 h before harvesting and staining for FITC-conjugated AnnexinV.