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. 2009 May 6;9:54. doi: 10.1186/1471-2334-9-54

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Copyright ©2009 Xu et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Analysis of cytokine production. Spleen cells were harvest from mice immunized with the SjGP-3 formulated by various adjuvants (three mice each group) on week 9 (before challenge), 12 (three weeks after cercarial challenge) and 15 (six weeks after cercarial challenge), and stimulated in vitro with anti-CD3, SjGP-3 protein or medium in duplicate wells respectively. The IL-4 and IFN-γ production in the culture supernatants of spleen cells was analyzed by ELISA in triplicate wells. Data correspond to the mean values ± standard deviation. (A) Week 9. (B) Week 12. (C) Week 15. * p < 0.05, ** p < 0.01 when compared with PBS control group.