Table 2.
Effects of immunization with the SjGP-3 formulated by various adjuvants on induction of IgG and protective efficacy against challenge in mice
| Groups a | IgG titers b | Worm burdens Mean ± SE |
%Worm reduction | Liver eggs (epg) Mean ± SE c |
% Egg reduction | |
|---|---|---|---|---|---|---|
| Exp1 | SjGP-3/CFA | 1,890,000 | 19.8 ± 0.9 | 41.1* | 20,900 ± 1,947 | 26.6* |
| SjGP-3/ISA206 | 1,290,000 | 23.4 ± 1.4 | 30.4* | 21,200 ± 2,307 | 25.6* | |
| SjGP-3/IMS1312 | 680,000 | 25.3 ± 1.2 | 24.7* | 24,400 ± 2,604 | 14.4 | |
| SjGP-3/ISA70M | 1,790,000 | 27.8 ± 1.2 | 17.3* | 15,100 ± 1,578 | 47.0*,# | |
| Control | < 500 | 33.6 ± 1.0 | / | 28,500 ± 2,777 | / | |
| Exp2 | SjGP-3/CFA | 1,831,000 | 20.9 ± 1.2 | 38.2* | 26,900 ± 2,557 | 29.6* |
| SjGP-3/ISA206 | 1,160,000 | 24.0 ± 1.6 | 29.0* | 28,300 ± 1,808 | 25.9* | |
| SjGP-3/IMS1312 | 783,000 | 24.7 ± 1.9 | 26.9* | 31,500 ± 2,593 | 17.5 | |
| SjGP-3/ISA70M | 1,841,000 | 26.0 ± 0.9 | 23.1* | 19,000 ± 1,817 | 50.3*,# | |
| Control | < 500 | 33.8 ± 0.8 | / | 38,200 ± 3,428 | / | |
a: The number of mice per group is from 8 to 10; b:Cut-off value was determined based on the pre-immunized geometric mean and three standard deviations. c: Number of liver eggs was calculated as egg numbers per gram of liver (epg). *: p < 0.05 when compared with PBS control group. #: p < 0.05 when compared with other adjuvant groups. Each value was derived from the arithmetic means of individuals in the group.