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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Biomaterials. 2008 Apr 8;29(19):2884–2890. doi: 10.1016/j.biomaterials.2008.03.026

Figure 2.

Figure 2

In vitro bioactivity of VEGF as a function of HUVEC proliferation over 72 hrs. Untreated cells did not demonstrate proliferation in serum-free media. HUVECs cultured in serum-free medium supplemented with 10 ng/ml of exogenous VEGF demonstrated an increase in proliferation at all timepoints. HUVECs exposed to serum-free media conditioned with VEGF-loaded microspheres (VEGFMS) had a statistically significant increase in proliferation up to 14 days after conditioning. This was not observed in HUVECs exposed to serum-free media conditioned with empty microspheres (Empty MS) (n=6/group, *p<0.05 compared to untreated group)