Table 1.
Phenotypic comparisons of SUMO isopeptidases vs. STUbLs
| ulp2Δ (Ulp1 mut) | STUbLts/Δ | References | ||
|---|---|---|---|---|
| SUMO pathway interaction | Accumulation of SUMO chains | Y (ulp2Δ) (S.c., S.p.) | Y (S.c., S.p.) | [11, 14, 15, 25, 92] |
| Growth defects suppressed by SUMO mutants defective for chain formation | Y (ulp2Δ) (S.c.) | N (S.c.) | [16, 25] | |
| Growth phenotype suppressed by deleting SUMO E3 ligase | Y (ulp2Δ) (S.c.) | N (S.c.) Y (S.p.) |
[11, 15, 25] | |
| Growth phenotype suppressed by over-expressing SUMO E3 ligase | Y (ulp2Δ2) (S.c.) | n.d. | [93] | |
| UV sensitivity suppressed by ULP2 overexpression | n.d./n.a. | Y (S.p.) | [14] | |
| Synthetic lethal with STUbL | Y (ulp1ΔN338) (S.c.) | n.a. | [27] | |
| High copy suppressor of ulp1ts | N (S.c.) | Y (S.c.) | [17] | |
| DNA damage sensitivity | HU Sensitivity | Y (ulp2Δ, ulp1ts) (S.c.) | Y (S.p., S.c.) | [10, 11, 92, 94] |
| MMS sensitivity | Y (ulp2Δ, ulp1ts) (S.c.) | N (S.c.) Y (S.p.) |
[11, 28, 92] | |
| UV sensitivity | Y (ulp2Δ, ulp1ts) (S.c.) | Y (S.c., S.p.) | [11, 17, 92, 95] | |
| CPT sensitivity | n.d. | N (S.c.) Y (S.p.) |
[11, 28] | |
| Genomic instability | Elevated DNA repair foci | Y (ulp1ts, ulp1ΔN338) (S.c.) | Y (S.c., S.p.) | [11, 27, 28, 95, 96] |
| Elevated spontaneous GCR | n.d. | Y (mainly telomere addition) (S.c.) | [28] | |
| Elevated 2μm plasmid number | Y (ulp1T490L, ulp1ΔES) (S.c.) | Y (S.c.) | [27, 97] | |
| Elevated chromosome & centromeric plasmid loss | Y (ulp2Δ2, ulp2Δ) (S.c.) | n.d. | [92, 93] | |
| Hyper-recombination | Y (ulp1ΔN338, ulp1I615N) (S.c.) | Y (S.c.) | [27, 95, 96] | |
| Elevated mutation rate | Y (ulp1I615N) (S.c.) | Y (S.c.) | [28, 95] | |
| Telomeric and rDNA silencing defect | Y and n.d. (ulp2Δ) (S.c.) | Y (S.c.) | [73] | |
| Role in telomere maintenance | Y (ulp1Δ160) | Y (S.c.) | [59, 98] | |
| Meiotic defects | Sporulation defect | Y (ulp2Δ) (S.c.) | Y (S.c.) | [10, 92] |
| Cell cycle progression | Growth reduced with G2/M arrest | Y (ulp1ts, ulp1I615N, ulp1ΔES) (S.c.) | Y (S.c., S.p.) | [11, 95, 97, 99] |
| Checkpoint constitutively activated | n.d. | Y (S.c.) | [28] | |
| Impaired checkpoint recovery/adaptation | Y (S.c.) | n.d. | [92, 94] | |
| Key genetic interactions | Suppressor of mot1ts | Y (ulp1, ulp2) (S.c.) | Y (S.c.) | [15] |
| High-copy suppressor of Smc2-6 | Y (ULP2) (S.c.) | N (S.c.) | [93] | |
| High-copy suppressor of pds5-1 | Y | N | [100] | |
| Synthetic lethal with srs2Δ | Y (ulp1I615N) (S.c.) | N (S.c.) | [95, 101] | |
| Synthetic lethal with sgs1Δ/rqh1Δ | N (S.c.) | Y (S.c., S.p.) | [10, 11] | |
| Synthetic lethal with mus81Δ/eme1Δ | N | N (S.c.) Y (S.p.) | [11, 28] | |
| Synthetic lethal/sick with recombination mutants rad51/52/54/55/50Δ or mre11Δ | Y (ulp1I615N) (S.c.) | Y (S.p.: rad51/55Δ and n.d.; S.c.1) | [11, 48, 95, 101] | |
| Synthetic sick with sir2/4Δ | Y (ulp2Δ) (S.c.) | Y (S.c.) | [73] | |
| Other | Microtubule destabilizing agent sensitivity | Y (ulp2Δ (S.c.) | Y (S.p.) | [92] & our unpublished results |
| Aberrant mitotic spindles | Y (ulp2Δ) (S.c.) | n.d. | [92] | |
| Temperature sensitivity | Y(ulp2Δ, ulp1I615N) (S.c.) | n.d. | [92, 95] | |
| Localization | Nuclear | Y Nuclear (Ulp2) and Nuclear pores (Ulp1) (S.c.) | Y (S.c., S.p.) Slx8 foci formation during S/G2 phase, colocalizing with PCNA and nucleolar protein Not1 (S.c.) | [11, 27, 93, 102, 103] |
1
Burgess et al. reported suppression of the nibbled growth phenotype in slx8Δ cells by deletion of homologous recombination enzymes Rad50, 52, 59 and Mre11 [27]. (S.c.), S. cerevisiae; (S.p.), S. pombe; n.a., not applicable; n.d., not done; Y, yes; N, no.