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OxyR activity in thioredoxin and glutaredoxin mutants. (A) Total RNA was isolated from untreated and hydrogen peroxide (200 μM)-treated wild-type (DHB4), trxA− (WP570), trxA− gorA− (FÅ378), trxA− gshA− (WP612), trxA− grxA− (WP813), and katG− ahpCF− (FÅ369) cells, and the levels of the OxyS RNA were assayed by primer extension. (B) The levels of alkaline phosphatase activity in midlogarithmically growing cells were determined as described (7).
