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Purification of starfish GSS. (A) The final semimicrochromatography with a SMART system by using a reverse-phase semimicrocolumn (1.5 × 150 mm) at a flow rate of 0.1 mL/min. The activity was recovered at 37–39 min, as shown by the asterisk (*). The activity of this fraction is shown in the histogram in the bottom half of the graph. (B) Mass spectrogram of purified starfish GSS after DTT treatment.
