Fig. 4.

Effects of crude radial nerve extract (A and B) and synthetic GSS (C and D) on cAMP and 1-MeAde production on starfish ovarian follicle cells. (A and C) Intracellular cAMP content. The amount of cAMP was measured by an EIA system. (B and D) 1-MeAde content produced by follicle cells. The amount of 1-MeAde released into the medium was estimated by in vitro maturation assays by using isolated oocytes, with an analytical curve generated with authentic 1-MeAde. Symbols and bars represent the mean ± SEM for 4 experiments.