Fig. 4.

The ability of HS to inhibit responses associated with PMN transmigration is not caused by downregulation of CD11b. Isolated PMN were incubated in HS (40 mM hypertonicity) or HBSS and stimulated with fMLP (10⁻⁶ M) for 20 min. CD11b expression on unstimulated cells (PMN) was compared with that on fMLP-stimulated PMN (fMLP) or PMN coincubated with fMLP and HS (fMLP + HS), as determined by fluorescence-activated cell sorter (FACS) analysis. MCF, mean channel fluorescence. *Significant differences between both fMLP-treated groups. **P < 0.01 by ANOVA, means ± SE, n = 5.