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. 2009 Feb 6;296(5):H1312–H1320. doi: 10.1152/ajpheart.00977.2008

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Copyright © 2009, American Physiological Society

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Fig. 1. — The measurement of hypoxia-induced pulmonary hypertension in rats. Immunostaining for α-smooth muscle actin demonstrated muscularization of pulmonary arteries (PAs) in sections from chronically hypoxic rats compared with normotensive rats (A). Quantification of thickening of the PA wall showed muscularization in chronically hypoxic lung sections immunostained for α-smooth muscle actin compared with normotensive lungs (B). Chronically hypoxic rats had increased right ventricular weight (RV)/left ventricular weight (LV) + septum weight (S) ratio (C) and hematocrit (D) compared with control animals. Results are expressed as means ± SE; n = 6 experiments. Scale bar = 50 μm. *P < 0.001 vs. control.