Fig. 4.

Effect of OxySpin on the differentiation of MSCs in osteogenic and adipogenic lineages. A and B are unlabeled and labeled MSCs induced to differentiate into adipocytes, respectively. Lipid vacuoles were formed after the 21-day process of induction, which was stained red by the Oil O red stain. C and D are unlabeled and labeled MSCs induced to differentiate into osteoblasts. Alizarin red stained the calcium deposition occurring in the process orange red. The arrows point to the OxySpin deposition in the cells. The results demonstrate that differentiation of MSCs into bone cells or fat cells was not affected by OxySpin.