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. 2009 Mar 13;296(5):H1263–H1273. doi: 10.1152/ajpheart.01311.2008

Fig. 5.

Fig. 5.

Effect of OxySpin on the differentiation of MSCs into cardiac and endothelial lineages. A: MSCs unlabeled (control) and labeled with OxySpin were induced to differentiate into cardiomyocyte-like cells using 5-azacytidine. Immunofluorescence staining for cardiac markers heavy chain (HC) cardiac myosin (green) and connexin-43 (red), superimposed on 4′,6-diamidino-2-phenylindole (DAPI) staining for nucleus (blue). B: expressions of endothelial markers CD31 (green) and von Willebrand Factor (vWF; red) are shown along with DAPI staining for nucleus (blue) in unlabeled (control) and labeled MSCs after 3 wk of culture in complete endothelial medium.