Fig. 4.

Oxidized low-density lipoprotein (oxLDL)-induced Ca²⁺ release in endothelial cells from B6 mice. Left: changes over time in cytosolic Ca²⁺ levels after stimulation with various doses of oxLDL. The increase in the ratio of fluorescence at wave length 340 nm to 380 nm indicates a rise in intracellular free Ca²⁺. oxLDL induced a dose-dependent increase in Ca²⁺ levels. Right: confocal microscopy images showing changes in intracellular distribution of Ca²⁺ labeled with fluorescent dye fura 2 at 0, 20, 60, and 120 s in endothelial cells from B6 mice after addition of oxLDL.