Table 2.
Pathways detected by Ingenuity pathway analysis of genes significantly differentially expressed in the aortic wall of B6.apoE−/− and C3H.apoE−/− mice fed a chow or Western diet
| Pathway Name | Gene Symbol | Gene Name | Affymetrix ID | Fold Change In | Fold Change P | Gene Product Location | Gene Ontology Type | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chow diet | ||||||||||||||
| Calcium signaling | ACTC1 | Actin, α, cardiac muscle 1 | 1415927_at | 25.992 | 0.000020 | Cytoplasm | Other | |||||||
| MEF2C | Myocyte enhancer factor 2C | 1421028_a_at | 9.19 | 0.000101 | Nucleus | Transcription regulator | ||||||||
| MYH6 | Myosin, heavy chain 6, cardiac muscle, α (cardiomyopathy, hypertrophic 1) | 1448554_s_at | 157.586 | 0.000020 | Cytoplasm | Enzyme | ||||||||
| MYL3 | Myosin, light chain 3, alkali; ventricular, skeletal, slow | 1427768_s_at | 6.063 | 0.000618 | Cytoplasm | Other | ||||||||
| MYL4 | Myosin, light chain 4, alkali; atrial, embryonic | 1422580_at | 59.714 | 0.000052 | Cytoplasm | Other | ||||||||
| MYL7 | Myosin, light chain 7, regulatory | 1449071_at | 194.012 | 0.000020 | Cytoplasm | Enzyme | ||||||||
| RCAN2 | Regulator of calcineurin 2 | 1450243_a_at | 12.126 | 0.000046 | Unknown | Other | ||||||||
| RYR2 | Ryanodine receptor 2 (cardiac) | 1450123_at | 9.849 | 0.000552 | Plasma membrane | Ion channel | ||||||||
| TNNC1 | Troponin C type 1 (slow) | 1418370_at | 8.574 | 0.000068 | Cytoplasm | Other | ||||||||
| TNNI3 | Troponin I type 3 (cardiac) | 1422536_at | 24.251 | 0.000020 | Cytoplasm | Transporter | ||||||||
| TNNT2 | Troponin T type 2 (cardiac) | 1424967_x_at | 181.019 | 0.000020 | Cytoplasm | Other | ||||||||
| Butanoate metabolism | ALDH1A1 | Aldehyde dehydrogenase 1 family, member A1 | 1416468_at | 9.19 | 0.002250 | Cytoplasm | Enzyme | |||||||
| HMGCS2 | 3-Hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial) | 1423858_a_at | 45.255 | 0.000030 | Cytoplasm | Enzyme | ||||||||
| MYO5B | Myosin VB | 1452298_a_at | −7.464 | 0.001486 | Cytoplasm | Enzyme | ||||||||
| Acute phase response signaling | HAMP | Hepcidin antimicrobial peptide | 1419197_x_at | 19.698 | 0.000078 | Extracellular space | Other | |||||||
| IL6ST | Interleukin 6 signal transducer (gp130, oncostatin M receptor) | 1421239_at | 12.996 | 0.000189 | Plasma membrane | Transmembrane receptor | ||||||||
| RBP7 | Retinol binding protein 7, cellular | 1449461_at | −36.758 | 0.000020 | Cytoplasm | Other | ||||||||
| SOCS3 | Suppressor of cytokine signaling 3 | 1455899_x_at | 32 | 0.000618 | Cytoplasm | Other | ||||||||
| TRF | Transferrin | 1425546_a_at | −12.996 | 0.000030 | Extracellular space | Transporter | ||||||||
| Nitric oxide signaling in the cardiovascular system | GUCY1A3 | Guanylate cyclase 1, soluble, α3 | 1420534_at | 12.996 | 0.000020 | Cytoplasm | Enzyme | |||||||
| PLN | Phospholamban | 1460332_at | 11.314 | 0.000130 | Cytoplasm | Other | ||||||||
| RYR2 | Ryanodine receptor 2 (cardiac) | 1450123_at | 9.849 | 0.000552 | Plasma membrane | Ion channel | ||||||||
| Hepatic fibrosis | COL1A2 | Collagen, type I, α2 | 1423110_at | 6.063 | 0.000020 | Extracellular space | Other | |||||||
| MYH6 | Myosin, heavy chain 6, cardiac muscle, α (cardiomyopathy, hypertrophic 1) | 1448554_s_at | 157.586 | 0.000020 | Cytoplasm | Enzyme | ||||||||
| MYL3 | Myosin, light chain 3, alkali; ventricular, skeletal, slow | 1427768_s_at | 6.063 | 0.000618 | Cytoplasm | Other | ||||||||
| MYL4 | Myosin, light chain 4, alkali; atrial, embryonic | 1422580_at | 59.714 | 0.000052 | Cytoplasm | Other | ||||||||
| MYL7 | Myosin, light chain 7, regulatory | 1449071_at | 194.012 | 0.000020 | Cytoplasm | Enzyme | ||||||||
| Western diet | ||||||||||||||
| Calcium signaling | ACTC1 | Actin, α, cardiac muscle 1 | 1415927_at | 68.594 | 0.00002 | Cytoplasm | Other | |||||||
| ATP2A2 | ATPase, Ca2+ transporting, cardiac muscle, slow twitch 2 | 1452363_a_at | 3.732 | 0.00003 | Cytoplasm | Transporter | ||||||||
| CASQ2 | Calsequestrin 2 (cardiac muscle) | 1422529_s_at | 3.482 | 0.001486 | Cytoplasm | Other | ||||||||
| CHRNA3 | Cholinergic receptor, nicotinic, α3 | 1452010_at | 16 | 0.000618 | Plasma membrane | Transmembrane receptor | ||||||||
| MYH6 | Myosin, heavy chain 6, cardiac muscle, α (cardiomyopathy, hypertrophic 1) | 1448554_s_at | 119.428 | 0.000023 | Cytoplasm | Enzyme | ||||||||
| MYL4 | Myosin, light chain 4, alkali; atrial, embryonic | 1422580_at | 16 | 0.00002 | Cytoplasm | Other | ||||||||
| MYL7 | Myosin, light chain 7, regulatory | 1449071_at | 128 | 0.00002 | Cytoplasm | Enzyme | ||||||||
| NFATC2 | Nuclear factor of activated T cells, cytoplasmic, calcineurin-dependent 2 | 1425901_at | −8.574 | 0.00107700 | Nucleus | Transcription regulator | ||||||||
| RYR2 | Ryanodine receptor 2 (cardiac) | 1450123_at | 2.83 | 0.003355 | Plasma membrane | Ion channel | ||||||||
| TNNC1 | Troponin C type 1 (slow) | 1418370_at | 10.556 | 0.00013 | Cytoplasm | Other | ||||||||
| TNNI3 | Troponin I type 3 (cardiac) | 1422536_at | 137.187 | 0.00002 | Cytoplasm | Transporter | ||||||||
| TNNT2 | Troponin T type 2 (cardiac) | 1424967_x_at | 147.033 | 0.00002 | Cytoplasm | Other | ||||||||
| Phenylalanine metabolism | GOT1 | Glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) | 1450970_at | 3.732 | 0.003355 | Cytoplasm | Enzyme | |||||||
| GOT2 | Glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) | 1417716_at | 4 | 0.001077 | Cytoplasm | Enzyme | ||||||||
| PRDX2 | Peroxiredoxin 2 | 1430979_a_at | 11.314 | 0.00225 | Cytoplasm | Enzyme | ||||||||
| Glutamate metabolism | GOT1 | Glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) | 1450970_at | 3.732 | 0.003355 | Cytoplasm | Enzyme | |||||||
| GOT2 | Glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) | 1417716_at | 4 | 0.001077 | Cytoplasm | Enzyme | ||||||||
| GPT2 | Glutamic pyruvate transaminase (alanine aminotransferase) 2 | 1455007_s_at | −45.255 | 0.00003000 | Unknown | Enzyme | ||||||||
| Arginine and proline metabolism | CKMT2 | Creatine kinase, mitochondrial 2 (sarcomeric) | 1428722_at | 13.929 | 0.000046 | Cytoplasm | Kinase | |||||||
| GAMT | Guanidinoacetate N-methyltransferase | 1422558_at | −12.126 | 0.00249000 | Cytoplasm | Enzyme | ||||||||
| GOT1 | Glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) | 1450970_at | 3.732 | 0.003355 | Cytoplasm | Enzyme | ||||||||
| GOT2 | Glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) | 1417716_at | 4 | 0.001077 | Cytoplasm | Enzyme | ||||||||
| Alanine and aspartate metabolism | GOT1 | Glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) | 1450970_at | 3.732 | 0.003355 | Cytoplasm | Enzyme | |||||||
| GOT2 | Glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) | 1417716_at | 4 | 0.001077 | Cytoplasm | Enzyme | ||||||||
| GPT2 | Glutamic pyruvate transaminase (alanine aminotransferase) 2 | 1455007_s_at | −45.255 | 0.00003000 | Unknown | Enzyme | ||||||||
| GOT1 | Glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) | 1450970_at | 3.732 | 0.003355 | Cytoplasm | Enzyme | ||||||||
| GOT2 | Glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) | 1417716_at | 4 | 0.001077 | Cytoplasm | Enzyme | ||||||||
| Parkinson's signaling | MAPK14 | Mitogen-activated protein kinase 14 | 1416704_at | −6.063 | 0.00304100 | Cytoplasm | Kinase | |||||||
| SNCA | Synuclein, α (non-A4 component of amyloid precursor) | 1436853_a_at | −6.964 | 0.00011400 | Cytoplasm | Other | ||||||||
| Cysteine metabolism | GOT1 | Glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) | 1450970_at | 3.732 | 0.003355 | Cytoplasm | Enzyme | |||||||
| GOT2 | Glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) | 1417716_at | 4 | 0.001077 | Cytoplasm | Enzyme | ||||||||
| HS3ST1 | Heparan sulfate (glucosamine) 3-O-sulfotransferase 1 | 1423450_a_at | −3.482 | 0.00016700 | Cytoplasm | Enzyme | ||||||||
| BMP signaling pathway | BMPR2 | Bone morphogenetic protein receptor, type II (serine/threonine kinase) | 1419616_at | −17.148 | 0.00034600 | Plasma membrane | Kinase | |||||||
| EGFR | Epidermal growth factor receptor [erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian] | 1424932_at | 7.464 | 0.003355 | Plasma membrane | Kinase | ||||||||
| MAPK14 | Mitogen-activated protein kinase 14 | 1416704_at | −6.063 | 0.00304100 | Cytoplasm | Kinase | ||||||||
| SMAD4 | SMAD family member 4 | 1422485_at | 3.249 | 0.00013 | Nucleus | Transcription regulator | ||||||||