Fig. 2.

Release of inflammatory chemokines by EPCs. Release of the chemokines monocyte chemoattractant protein-1 (MCP-1; A), interleukin-8 (IL-8; B), and regulated upon activation, normal T cell expressed and secreted (RANTES; C) by early EPCs (n = 4), adult blood late EPCs (n = 3), and cord blood late EPCs (n = 4) was measured over a 24-h period. The data for each chemokine are presented as means ± SE of secreted ng/million adherent cells. In A, the overall P value for comparative MCP-1 release by ANOVA was P < 0.01, but the only significant difference in the post hoc analysis was P < 0.01 (**) between early EPCs and adult late EPCs. In B, the overall ANOVA of the IL-8 release was highly significant (P < 0.005), and this was driven by the difference in IL-8 release between early EPCs and adult late EPCs (**P < 0.01) and by the difference between the cord blood late EPC group and adult late EPCs (**P < 0.01). In C, the overall ANOVA of the RANTES release was not significant (P = 0.095), so no post hoc analysis could be performed, although there was a trend toward higher RANTES release by early EPCs compared with either one of the late EPC groups.