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. 2009 Mar 13;296(5):H1466–H1483. doi: 10.1152/ajpheart.00795.2008

Table 1.

Primers used in RT-PCR analysis

Forward Primer Reverse Primer
iNOS 5′-ATTCACAGCTCATCCGGTACG-3′ 5′-GGATCTTGACCATCAGCTTGC-3′
eNOS 5′-CATTTTCGGACTCACATTGCG-3′ 5′-TTGGTCAACCGAACGAAGTG
nNOS 5′-CAGGCAAATCCCAAGCCTATG-3′ 5′-CAAAGCACAGCCGAATTTCTC-3′
NOX1 5′-TCGAACGCTACAGAAGAAGCC-3′ 5′-TGGCAATCACTCCAGTAAGGC-3′
NOX2 5′-GACCATTGCAAGTGAACACCC-3′ 5′-AAATGAAGTGGACTCCACGCG-3′
p22phox 5′-ATGGAGCGATGTGGACAGAAG-3′ 5′-TAGATCACACTGGCAATGGCC-3′
p40phox 5′-TTCAAAGACCTGCTAGCGCT-3′ 5′-TCCTTCTGTGTGACATGCAGC-3′
p47phox 5′-TTCCATCCCCAAATGCAAAG-3′ 5′-TCAGATGCCCTAAAACCGGAG-3′
p67phox 5′-GCCTTCACCAAAAGCATCAAC-3′ 5′-ACCTCACAGGCAAACAGCTTG-3′
Xanthine oxidase 5′-AAAGGACCAGACGATTGCTCC-3′ 5′-TCACACGTTCCCCTTCAAAAC-3′
MMP-2 5′-CAAGGACCGGTTTATTTGGC-3′ 5′-ATTCCCTGCGAAGAACACAGC-3′
MMP-9 5′-TCTTCTGGCGTGTGAGTTTCC-3′ 5′-CGGTTGAAGCAAAGAAGGAGC-3′
Caspase-3 5′-GGACTGTGGCATTGAGACAG-3′ 5′-CGACCCGTCCTTTGAATTTC-3′
Caspase-9 5′-GGATGCTGTGTCAAGTTTGCC-3′ 5′-CTTTCGCAGAAACAGCATTGG-3′
Actin 5′-TGCACCACCAACTGCTTAG-3′ 5′-GGATGCAGGGATGATGTTC-3′

iNOS, inducible nitric oxide synthase (NOS); eNOS, endothelial NOS; nNOS, neuronal NOS; NOX, NAD(P)H oxidase; MMP, matrix metalloproteinase.