Table 1.
Forward Primer | Reverse Primer | |
---|---|---|
iNOS | 5′-ATTCACAGCTCATCCGGTACG-3′ | 5′-GGATCTTGACCATCAGCTTGC-3′ |
eNOS | 5′-CATTTTCGGACTCACATTGCG-3′ | 5′-TTGGTCAACCGAACGAAGTG |
nNOS | 5′-CAGGCAAATCCCAAGCCTATG-3′ | 5′-CAAAGCACAGCCGAATTTCTC-3′ |
NOX1 | 5′-TCGAACGCTACAGAAGAAGCC-3′ | 5′-TGGCAATCACTCCAGTAAGGC-3′ |
NOX2 | 5′-GACCATTGCAAGTGAACACCC-3′ | 5′-AAATGAAGTGGACTCCACGCG-3′ |
p22phox | 5′-ATGGAGCGATGTGGACAGAAG-3′ | 5′-TAGATCACACTGGCAATGGCC-3′ |
p40phox | 5′-TTCAAAGACCTGCTAGCGCT-3′ | 5′-TCCTTCTGTGTGACATGCAGC-3′ |
p47phox | 5′-TTCCATCCCCAAATGCAAAG-3′ | 5′-TCAGATGCCCTAAAACCGGAG-3′ |
p67phox | 5′-GCCTTCACCAAAAGCATCAAC-3′ | 5′-ACCTCACAGGCAAACAGCTTG-3′ |
Xanthine oxidase | 5′-AAAGGACCAGACGATTGCTCC-3′ | 5′-TCACACGTTCCCCTTCAAAAC-3′ |
MMP-2 | 5′-CAAGGACCGGTTTATTTGGC-3′ | 5′-ATTCCCTGCGAAGAACACAGC-3′ |
MMP-9 | 5′-TCTTCTGGCGTGTGAGTTTCC-3′ | 5′-CGGTTGAAGCAAAGAAGGAGC-3′ |
Caspase-3 | 5′-GGACTGTGGCATTGAGACAG-3′ | 5′-CGACCCGTCCTTTGAATTTC-3′ |
Caspase-9 | 5′-GGATGCTGTGTCAAGTTTGCC-3′ | 5′-CTTTCGCAGAAACAGCATTGG-3′ |
Actin | 5′-TGCACCACCAACTGCTTAG-3′ | 5′-GGATGCAGGGATGATGTTC-3′ |
iNOS, inducible nitric oxide synthase (NOS); eNOS, endothelial NOS; nNOS, neuronal NOS; NOX, NAD(P)H oxidase; MMP, matrix metalloproteinase.