Table 2.

Protein sequence features related to subcellular localization prediction.

	Secretion System and/or SP
TOOLS	SPI	SPII	Cyt	TM	Type I	Type II	Cell Wall	Extracell	Plasma membrane	Periplasm
SignalP 2.0 (Sec-dependent)	X							X	X
TatP 1.0 (altern system)	X						X	X	X
LipoP 1.0 (Sec-dependent)	X	X	X	X				X	X
Phobius				X	X	X
PA-SUB 2.5	X	X	X
Gpos-Ploc			X				X	X	X	X
PSORTb v.2.0.4			X					X	X	X

SP, Signal Peptidase. SPI, Signal Peptidase I. SPII, Signal Peptidase II. Cyt, Cytoplasmatic. TM, Transmembranal protein.