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. 2009 May 8;9:86. doi: 10.1186/1471-2180-9-86

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Copyright ©2009 Moolhuijzen et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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PCR assay specificity for C. fetus subspecies and C. fetus subsp veneralis. Examples of PCR assay specificity for C. fetus subspecies and C. fetus subsp veneralis biovars (venerealis and intermedius). Lanes numbered 1–4, N and M represent: 1 Cfv biovar venerealis 19438 ATCC, 2 Cfv biovar intermedius (Pfizer strain), 3 Cfv Argentina AZUL-94 strain, 4 Cff 15296 ATCC, N= negative no template control and M = molecular weight marker 100 bp ladder (Invitrogen). Results are shown for assays based on Contig1154 orf3 (429 bp), Contig 1165 orf4 (233 bp), Contig 1023 orf2 (159 bp) and Contig1023 orf3 (349 bp).