Figure 6.
Real time qRT-PCR results for nine selected genes from trypanotolerant N'Dama and trypanosusceptible Boran cattle across the trypanosome infection time course. Data analysis was carried out using the standard 2-ΔΔCt method [55]. All Ct values were corrected using the housekeeping gene PPIA and the N'Dama group at time point 0 was used as the calibrator. Each point on the graph (common log scale) represents the mean fold change in gene expression relative to pre-infection levels ± SEM. Genes shown are: RAB35, NFE2L2, GZMB, LYZ, XDH, GBP4, CTSS, NCR3 and BAFF.