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. 2009 Jun;11(6):509–517. doi: 10.1593/neo.81578

Figure 2.

Figure 2

EphA2 antibodies elicit ADCC activity in vitro. The ADCC activity of 3F2-3M was assessed using healthy donor PBMCs as effectors and SKOV3 ovarian cancer (A), A549 NSCLC (B) and MDA-MB-231 breast cancer (C) cell lines as targets as described in Materials and Methods. Cytotoxicity was observed in a dose-dependent and effector-to-target ratio manner. Concentrations of 3F2-3M or isotype control antibodies used were 5 ng/ml (diamonds), 50 ng/ml (hatched lines), or 500 ng/ml (solid bars). Little to no cytotoxicity was observed when 3F2-3M was evaluated in the SKMel28, a non-EphA2-expressing melanoma cell line (D). Error bars, SEM. Data are representative of three independent experiments.