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. 2009 Jun;11(6):509–517. doi: 10.1593/neo.81578

Figure 4.

Figure 4

CD107a expression is increased on NK cells in the presence of EphA2 antibodies. The expression of the activation marker CD107a was evaluated on the surface of healthy donor NK cells by flow cytometry. Isolated NK or non-NK cells were incubated in the presence of tumor cells expressing or lacking EphA2, EphA2 antibodies, or isotype control antibodies. When EphA2-expressing tumor cells are present, a dose-responsive increase in CD107a protein occurs with both the 3F2-WT (open squares) and 3F2-3M (solid squares) antibodies, where 3F2-3M > 3F2-WT. At high concentrations of antibody, CD107a expression increases in a nonspecific fashion as observed with the isotype control antibody (open circles) and with EphA2-negative cell lines (data not shown). Error bars, SEM. 3F2-3M was statistically different from both 3F2-WT and isotype control antibody at all concentrations greater than 0.010 µg/ml (P < .001).