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OSU-HDAC42-induced epithelial differentiation of ovarian cancer cells. (A) Dose-dependent morphologic changes after a 24-hour OSU-HDAC42 treatment of A2780 (upper panel) and CP70 (lower panel) cells. (B) Up-regulation of numerous cytokeratins (as assessed by AE1/AE3 monoclonal antibody cocktail staining) in A2780 (upper panel) and CP70 (lower panel) cells after a 48-hour OSU-HDAC42 treatment. Inset numbers: flow cytometric quantification of AE1/AE3-positive CP70 cells.
