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. 2009 Jun;11(6):574–582. doi: 10.1593/neo.09254

Figure 1.

Figure 1

Flow cytometric analysis of EL-4 cells treated for 16 hours with etoposide. (A) Detection of apoptotic cells using annexin V—Pacific Blue (λ Ex/Em = 410/455) and necrotic cells by SYTOX Red nuclear staining (λ Ex/Em = 640/658). (B) Changes in cellular autofluorescence after treatment. The autofluorescence observed at 455 nm with excitation at 350 nm has been assigned to NADH [25,26]. Population 1 represents viable cells, whereas population 2 represents apoptotic cells that have a low UV autofluorescence and stain with annexin V. Population 3 represents necrotic cells.