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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: J Immunol. 2009 Jun 1;182(11):6670–6681. doi: 10.4049/jimmunol.0803683

Figure 14. In vitro.

Figure 14

IgM anti-HBc antibody production requires 3 signals. TCR-Tg 7/16-5 spleen cells were cultured with varying concentrations of HBcAg preparations, which contained LPS (10 ng/μg) HBcAg149 RNA−/LPS+; ssRNA, HBcAg183 RNA+/LPS−; neither LPS or ssRNA, HBcAg149 RNA−/LPS− or LPS without HBcAg particles. After 5 days of culture, supernatants (SN) were collected and IgM anti-HBc antibodies measured by ELISA. This experiment was performed on 3 separate occasions and the results are representative.