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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: J Immunol. 2009 Jun 1;182(11):6670–6681. doi: 10.4049/jimmunol.0803683

Figure 2.

Figure 2

Efficient CD4+ T cell priming in vivo with soluble HBcAg requires B cells. Groups of 3 B10.S/+/+ or B10.S/BKO mice were immunized (i.p.) with HBcAg183 (10μg) emulsified in CFA. Ten days after immunization spleen cells were harvested, pooled and cultured with the indicated concentrations of a panel of recall antigens (HBcAg183, HBeAg and p120–140). Culture supernatants were collected at days 2 and 4 for the determination of IL-2 and IFNγ, respectively, by ELISA. Groups of 3 mice each were also immunized with the HBsAg (20 μg, CFA) and spleen cells were cultured with varying concentrations of HBsAg in vitro (INSET). This experiment was performed on at least 3 occasions and the results are representative.