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. Author manuscript; available in PMC: 2009 May 22.
Published in final edited form as: J Mol Med (Berl). 2008 Jul 4;86(10):1139–1152. doi: 10.1007/s00109-008-0374-7

Fig. 3.

Fig. 3

APECED patient derived moDCs respond poorly to microbial stimulus. MoDCs differentiated from APECED patients or control individuals were stimulated with C. albicans (MOI 1) or infected with Sendai virus (MOI 5); cells were collected at 24 h after microbial stimulation and total cellular RNA was isolated. Expression of cytokine genes relevant for moDCs were studied using QRT-PCR of unstimulated, Sendai virus infected, or C. albicans-stimulated moDCs from patients (gray bars) and from control individuals (white bars). Means of triplicate specimen and their standard deviations are shown using the delta CT method (controls n=6, patients n=2–5). The expression of cytokine mRNA is shown in relation to β-actin mRNA. Standard errors are shown and significance was calculated using Student's t-test