Fig. 4.

Human single nucleotide polymorphism C to T conversion in the 5′-untranslated region of SOD3 (rs699473) reduces nuclear protein-binding capacity. Nuclear extract was prepared from human NCI-H441 lung cells, and a competitive electrophoretic mobility shift assay was performed with excess double-stranded DNA that contained either the C or the T variants. The C variant was more effective in competing the high-molecular-weight protein (top arrow), whereas the other major proteins were not different (bottom 2 arrows) compared with the competition with the T variant. Gel is representative of samples run in duplicate on 3 occasions.