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Analysis of interactions between rpS4 and rpS5 mutants by Leucine protrophy. The fusion proteins encoded in the DNA-BD and AD vectors are shown on the right. Ten microlitres of fivefold serial dilutions of cells are spotted on SD/gal/raf plates with Leu (+Leu) and without Leu (−Leu) and incubated at 30°C for 5 days.
