FIGURE 3.

Depletion of core exosome components results in longer NAB2 mRNAs. (A) YSB3002 (tetO₇-RRP43) or YSB3003 (tetO₇-RRP46) was grown in YPD at 30°C and treated (+) or not (−) with doxycycline for 8 h after which total RNA was prepared and RNA levels determined by Northern blotting. (B,C) Northern blot analysis of total RNA after RNase H treatment as described in Materials and Methods using OSB313 and total RNA from strains (B) YSB3001 (wt), YSB3002 (tetO₇-RRP43), or YSB3003 (tetO₇-RRP46); and (C) CWO4 (WT), YSB214 (rrp6-Δ), MS157-1A (mtr4-1), or YRP1222 (rrp4-1).