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The TRAMP component Trf4p is required for normal 3′-end formation of NAB2 mRNA. (A) Northern blot analysis of total RNA from strains YSB1001 (RRP6), YSB1005 (rrp6-Δ), YSB1017 (trf4-Δ), YSB1020 (trf4-Δ), YSB1060 (air1-Δ), and YSB1061 (air2-Δ). (B) Northern blot analysis of total RNA from strains YSB3001 (WT) or YSB3017 (tetO7-RRP46) grown for 5 h in the presence of 10 μg/mL doxycycline.
