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The nab2-1 mutation bypasses the requirement for Trf4p in NAB2 mRNA 3′-end processing. Northern blot analysis of total RNA from strains YSB501 [nab2∷HIS3 pACY717 (NAB2 LEU2 CEN)], YSB503 [nab2∷HIS3 trf4∷KANMX pACY717 (NAB2 LEU2 CEN)], YSB502 [nab2∷HIS3 pACY1152 (nab2-1 LEU2 CEN)], and YSB504 [nab2∷HIS3 trf4∷KANMX pACY1152 (nab2-1 LEU2 CEN)] grown in YPD at 30°C.
