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. 2009 Jun;15(6):1078–1089. doi: 10.1261/rna.1363109

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FIGURE 3. — A conserved tryptophan residue is important for Ago2 binding. (A) Alignment of the highly conserved regions of human GW182 (AY035864), TNRC6B (AB029016), TNRC6C (NM_018996), TNRC6Bisoform2 (NM_001024843), Drosophila melanogaster (Dm) GW182 (NP_726596), Caenorhabditis elegans AIN-1 (NM_078286), and Schizosaccharomyces pombe Tas3 (O94687). Highly conserved amino acids are indicated in bold. (B) The partial amino acid sequences with the point mutations in GW182 445-526 (top), 716-806 (middle), and 778-910 (bottom). Mutated amino acid residues are indicated in bold. (C–E) SBP pull-down assays of Ago2 and GW182 mutants. HEK293F cells were cotransfected with FLAG-Ago2 and FLAG/SBP-GW182 mutant expression vectors. SBP pull-down assays and Western blotting were performed as shown in Figure 1C.